S O U R C E
OBTAIN CURRENT STATUS
Whatever your stated goals, it’s important to understand where PRRS has or has not been present in your herd. The testing of blood, oral fluids and the environment may be essential in obtaining your current status.
KNOW THE SYMPTOMS
Recognizing clinical signs of PRRS is important to spotting the disease early. Work with your herd veterinarian to further diagnose if any of these symptoms are present:
- Late-term abortions
- Premature farrowing
- Delivery of stillborn, weak/listless/non-viable or mummified piglets
- Anorexia and fever
- Sow mortality
- Fever, depression, reduced appetite
- Discharge from eyes and nose
- Respiratory symptoms; specifically, labored abdominal breathing often referred to as “thumping”
- Uneven growth, gaunt appearance
- Increased culls and mortality post-weaning
Which test is right for you?
PRRS infection is widespread in U.S. herds, so care must be taken to both confirm an active infection and to rule out other infectious diseases. Any tentative clinical diagnosis should be confirmed by detection of the PRRS virus. The most common diagnostic tests used for confirming PRRS include:
- Polymerase chain reaction (PCR) – Detects presence of virus
- ELISA – Detects presence of antibody following infection
- Gene sequencing – Differentiates PRRS virus strains
The primary sample types that can be collected and used for diagnostic testing include:
- Oral fluids
- Tissues (lung, tonsil and/or lymph node)
- Environmental samples
Sample Diagnostic Testing for Replacement Gilts:
Entry into Gilt Pool Exiting Gilt Pool/Prior to
Blood/Serum – PCR and ELISA Blood/Serum – PCR and ELISA Oral – PCR Oral – PCR
Sample Diagnostic Testing for Sows:
Routine Monitoring/Testing Due-to-Wean Piglets Blood/Serum – PCR
Testing Growing Pigs for PRRS
8–10 Weeks of Age
12–14 Weeks of Age
18–20 Weeks of Age
Blood/Serum – PCR and ELISA Blood/Serum – PCR and ELISA Blood/Serum – PCR and ELISA Oral – PCR Oral – PCR Oral – PCR
Tissue samples can be used to further confirm disease. Always work with your veterinarian when developing a diagnostic testing program and collecting samples.
Tips for Best Sample Collection
Detection of PRRS virus is best performed in affected pigs during the early stages of PRRS infection:
- Weak-born, lethargic newborn/neonatal pigs
- Weak, off-feed and feverish post-weaned pigs
- Sows/Gilts exhibiting clinical signs, recently aborted, feverish and/or off-feed
Good choices for diagnostic samples and tissues for virus detection methods include:
- Oral fluids
- Lung, lymph node, tonsil and/or spleen tissues
- Collect samples and immediately refrigerate/place on ice.
- Both fresh and formalin-fixed tissue samples, if possible
- Send samples overnight to a laboratory using an insulated box with ice packs.
- Completely fill out laboratory submission form.
HMC Diagnostics Lab
Boehringer Ingelheim Vetmedica, Inc.’s Health Management Center (HMC) is one of the leading swine-specific diagnostic laboratories in North America. HMC can conduct ELISA and PCR testing for PRRS, as well as provide a variety of other services. Talk to your veterinarian for advice on sampling.